Study on rat small intestinal absorption features of puerarin loaded O-carboxymethyl chitosan microspheres based on single pass perfusion method
10.7501/j.issn.0253-2670.2016.14.016
- Author:
Shi-Yu LIU
1
Author Information
1. Shaanxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Absorption rate constants;
Apparent absorption coefficient;
Gravimetric method;
Puerarin loaded O-carboxymethyl chitosan microspheres;
Single-pass intestine perfusion
- From:
Chinese Traditional and Herbal Drugs
2016;47(14):2488-2491
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the rat intestinal absorption characteristic of puerarin loaded O-carboxymethyl chitosan (CMCS) microspheres in situ. Methods: Single-pass intestinal perfusion (SPIP) model was used for rat in situ and HPLC to determine the concentration of puerarin. The effects of different perfusion rate, drug concentration, and intestinal segments on intestinal absorption were investigated, and the absorption characteristics of the puerarin and puerarin loaded O-CMCS microspheres were compared. Results: Perfusion rate had significant effect on the absorption rate constants (Ka) and the apparent absorption coefficient (Papp) (P < 0.05); The concentration of puerarin in perfusate had no significant effect on Ka and Papp (P > 0.05); Ka and Papp of drug loaded microspheres in jejunum and ileum showed no significant difference, but they were significantly higher than that in duodenum (P < 0.05); The drug loaded microspheres and puerarin had the significant difference in Ka and Papp in jejunum (P < 0.05). Conclusion: The absorption mechanism of puerarin in the microspheres is passive diffusion, puerarin is absorbed well in jejunum and ileum, and puerarin loaded O-CMCS microspheres can significantly improve the absorption of puerarin.
