Simultaneous determination of eight components in Shenwu Jiannao Capsules by HPLC-MS/MS
10.7501/j.issn.0253-2670.2016.14.013
- Author:
Yi ZHANG
1
Author Information
1. Department of Pharmacy, Tianjin Huanhu Hospital
- Publication Type:Journal Article
- Keywords:
Baicalin;
Calycosin-7-glucoside;
Ginsenoside Re;
Ginsenoside Rg1;
HPLC-MS/MS;
Multiple reaction monitoring;
Paeoniflorin;
Puerarin;
Shenwu Jiannao Capsules;
Stibene glucoside;
Tanshinone IIA
- From:
Chinese Traditional and Herbal Drugs
2016;47(14):2470-2474
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the simultaneous determination of eight components (puerarin, tanshinone IIA, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg1, calycosin-7-glucoside, and baicalin) in Shenwu Jiannao Capsules (SJC). Methods: Chromatographic column: YMC-Triart C18, mobile phase: methanol-water (including 0.2% formic acid), and gradient elution, flow rate: 0.6 mL/min, column temperature: 40℃. MS condition: electrospray ionization (ESI) and multiple reaction monitoring (MRM) were adopted, the detected peak areas of ion pairs were used for quantitative determination. Results: There was a good linearity between the absorption peak area and the concentration for puerarin, tanshinone IIA, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg1, calycosin-7- glucoside, and baicalin in the ranges of 20.31-20 310, 25.66-25 660, 20.45-20 450, 50.79-50 790, 50.57-50 570, 50.38-50 380, 10.32-10 320, and 25.74-25 740 ng/mL, respectively (r ≥ 0.999 0). The precision was good and RSD was less than 2.0%. The repeatability was good and RSD was less than 2.0%. The stability was good in 24 h. The average recoveries were ranged from 97.98% to 102.48% (RSD ≤ 1.6%). The contents of puerarin, tanshinone IIA, stibene glucoside, paeoniflorin, ginsenoside Re, ginsenoside Rg1, calycosin-7-glucoside, and baicalin in six batches of samples were in the ranges of 1.843-1.860, 1.618-1.629, 2.116-2.129, 2.537-2.547, 0.034-0.041, 0.048-0.055, 0.551-0.564, and 2.333-2.346 mg/g, respectively. Conclusion: The method is rapid, simple, repeatable, and can be used to determine the multi components in different batches of SJC simultaneously.
