LC-MS guided discovery, structural elucidation of a new curcuminoids, and its cytotoxicity
10.7501/j.issn.0253-2670.2016.14.004
- Author:
Rui LI
1
Author Information
1. School of Food and Bioengineering, Xihua University
- Publication Type:Journal Article
- Keywords:
Bisabola-sesquiterpene;
Cucurminoids;
Curcuma Longa Rhizoma;
Curcuterpene G;
Cytotoxicity;
Guided separation
- From:
Chinese Traditional and Herbal Drugs
2016;47(14):2418-2423
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Separation and purification of a new curcuminoid from the rhizome of Curcuma longa by guided separation method. Methods: The chemical constituent was separated and purified through various kinds of chromatographic methods, including MCI-Gel, ODS-C18 reverse phase column, and RP-HPLC. The structure was elucidated by physicochemical properties and spectral data. Results: A new curcuminoid, 3,4'''-epoxy-5'''-C-(1α,2β,3β-bisabola-4,10-diene-9-one)-(2→5''')-curcumin (1), was isolated from the low polar fraction of methanol extracts from the rhizome of C. longa, named as curcuterpene G. This compound exhibited the inhibitory effect to HepG2, A549, and CT-26 tumor cells with the IC50 values of 4.82, 8.81, and 5.40 μmol/L, respectively. Conclusion: A new curcuminoid is isolated from the rhizome of C. longa by guided discovery method, and it exhibits notable cytotoxic activities.
