Screening of reference genes in Lonicera macranthoides and spatio-temporal expression analysis of LmAGL15 in Mads-box family
10.7501/j.issn.0253-2670.2016.15.022
- Author:
Jia-Luo CAI
1
Author Information
1. The First Hospital of Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
18 S rRNA;
Actin;
AGL15;
Bud period extended;
Efl-β;
Lonicera macranthoides Hand.-Mazz;
Reference gene;
Spatio-temporal expression characteristics;
Ubiquilin
- From:
Chinese Traditional and Herbal Drugs
2016;47(15):2727-2733
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the reference genes of Lonicera macranthoides for gene expression analysis and to study the spatio-temporal expression characteristics of LmAGL15 which was a member of Mads-Box family. Methods: In this study, 18 S rRNA, Ubiquilin, Actin and Efl-β of L. macranthoides were cloned and the stabilities of the four housekeeping genes were evaluated in different positions (leaves, stems, and buds) and different periods of bud development. In addition, the spatio-temporal expression of LmAGL15 gene was analyzed. Results: 18 S rRNA was the most suitable reference gene for spatio-temporal expression analysis in L. macranthoides; The relative expression of LmAGL15 was low in leaves and stems, and that in buds was higher. Conclusion: 18 S rRNA is the most suitable reference gene in L. macranthoides. The relative expression of LmAGL15 changes significantly in leaves, stems, and buds.
