Determination of asperosaponin VI, psoralen, and angelicin in Xianling Gubao Capsule by HPLC
10.7501/j.issn.0253-2670.2016.16.015
- Author:
Qin-Dan GONG
1
Author Information
1. Jinhua Wucheng City Food and Drug Administration
- Publication Type:Journal Article
- Keywords:
Angelicin;
Asperosaponin VI;
Multi-wavelength HPLC method;
Psoralen;
Quantitative determination;
Xianling Gubao Capsule
- From:
Chinese Traditional and Herbal Drugs
2016;47(16):2868-2870
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine asperosaponin VI, psoralen, and angelicin in Xianling Gubao Capsules (XGC) via multi- wavelength HPLC method. Methods: Separation was carried out on Welch Ultimate® XB-C18 column. The mobile phase was acetonitrile-water system and a linear gradient elution was used. The column temperature was 30℃. The detection wavelength for asperosaponin VI was set at 212 nm, those for psoralen and angelicin were set at 246 nm. Results: Three components reached baseline separation, the linearity was good when sample volumes were in the ranges of 144.1-5 764.0 for asperosaponin VI (r = 0.999 6), 5.4-215.2 (r = 0.998 0) for psoralen, and 6.6-265.6 ng (r = 0.998 5) for angelicin. The average recoveries of asperosaponin VI, psoralen, and psoralen were 98.11%, 97.86%, and 98.22%, respectively. The RSDs of recoveries were all less than 2.0%. Conclusion: The method is simple and accurate and has good separation, with high sensitivity and good efficiency for the determination of more-index components in XGC.
