Construction of CHI gene expression vector of safflower and its transformation in Arabidopsis thaliana
10.7501/j.issn.0253-2670.2016.18.023
- Author:
Xiu-Ming LIU
1
Author Information
1. Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Arabidopsis thaliana L.;
Carthamus tinctorius L.;
Chalcone isomerase;
Flavone;
PCR
- From:
Chinese Traditional and Herbal Drugs
2016;47(18):3279-3283
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Plant expression vector of chalcone isomerase in safflower was built and its function was verified by over- expression of CHI in Arabidopsis thaliana. Methods: CHI isolated from safflower in our previous study was introduced by BamH I and EcoR I restriction sites and constructed into the over-expression vector pBASTA-CHI containing 35S promoter, transformed into A. thaliana by Flora-dip method. T2 plants of transgenic A. thaliana were detected by PCR and content of total flavones. Results: Plant expression vector containing the safflower CHI gene was built and over expressed in A. thaliana successfully. T2 plants of transgenic A. thaliana were obtained. Conclusion: PCR of transgenic T2 in A. thaliana is detected that CHI gene of safflower has been integrated into the A. thaliana genome, flavone content is determined in leaves, and the results show that the flavone in transgenetic A. thaliana is higher than that in wild type, the highest strain increases by nearly 2.3 times.
