Preparation of rare ginsenoside by transformation of ginsenoside Re catalyzed with aspartic acid
10.7501/j.issn.0253-2670.2016.19.007
- Author:
Juan XIA
1
Author Information
1. Institute of Agricultural Modernization, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Acid hydrolysis;
Aspartic acid;
Ginsenoside F4;
Ginsenoside Re;
Ginsenoside Rg6;
Ginsenoside Rh4;
Ginsenoside Rk3;
Rare ginsenoside;
Semi preparative high performance liquid chromatography
- From:
Chinese Traditional and Herbal Drugs
2016;47(19):3389-3394
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A new, environment-friendly and efficient method for the preparation of rare ginsenoside Rg6, F4, Rk3, and Rh4 was established, which provides a theoretical basis for preparing rare ginsenosides. Methods: Rare ginsenoside was prepared by hydrolyzing ginsenoside Re using aspartic acid as the catalyst, through semi preparative HPLC, the target compounds Rg6, F4, Rk3, and Rh4 were rapidly separated from the degradation products, quantitative analysis, and structure identification by HPLC and NMR. Results: Ginsenoside Re was hydrolyzed by aspartic acid according to the ratio 10∶1 at 120℃ for 1 h, the conversion rate of ginsenoside Re was 100%, the yields of rare ginsenoside Rg6, F4, Rk3, and Rh4 were 11.2%, 13.1%, 20.6%, and 24.3%, respectively, and the purity of the four compounds were all above 99%. Conclusion: The method is simple, low-cost, and non-pollution for environment, the research has important application value for the development of green environmental protection of rare ginsenosides drugs and health food.
