Study on substance basis of Yinhuang Qingfei Capsule in vitro by HPLC-Q-TOF-MS/MS
10.7501/j.issn.0253-2670.2016.20.007
- Author:
Qing-Yi-Jun ZHOU
1
Author Information
1. Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Amygdalin;
Ephedrine;
Hesperidin;
HPLC-Q-TOF-MS/MS;
Peimine;
Quercetin;
Yinhuang Qingfei Capsule
- From:
Chinese Traditional and Herbal Drugs
2016;47(20):3586-3593
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify and analyze the chemical constituents in Yinhuang Qingfei Capsule by HPLC-Q-TOF-MS/MS method. Methods: The HPLC method was used with the conditions that the column was Inertsil ODS-2 C18 (250 mm × 4.6 mm, 5 μm). Columu and electrospray ion (ESI) source was employed for the qualitative analysis under positive ion mode. These components were further analyzed by MS spectra, and by comparing with the corresponding reference substances and literature data. Results: According to the MS principle and literature data, 54 compounds were identified from the sample of Yinhuang Qingfei Capsule. Conclusion: An efficient HPLC-Q-TOF-MS/MS approach has been established for studying the chemical constituents in Yinhuang Qingfei Capsule, which paves a way for the quality control and further substance basis studies of the preparation.
