Difference of chemical compositions in Xanthii Herba and Xanthii Fructus by UPLC-Triple TOF-MS/MS
10.7501/j.issn.0253-2670.2016.22.004
- Author:
Juan-Xiu LIU
1
Author Information
1. Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Partial least-squares discriminant analysis;
Principal component analysis;
UPLC-Triple TOF-MS/MS;
Xanthii Fructus;
Xanthii Herb
- From:
Chinese Traditional and Herbal Drugs
2016;47(22):3951-3958
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the difference of chemical compositions in Xanthii Herba and Xanthii Fructus. Methods: Eight batches of Xanthii Herba and Xanthii Fructus samples from different origins were determined by UPLC-Triple TOF-MS/MS. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, and noise filtering. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used for data processing. The components were identified according to a mass spectrometry accurate mass and two mass spectrometry fragmentation information, combined with the software of database search and literature. Results: The chemical compositions in Xanthii Herba and Xanthii Fructus samples are clearly distinguished. Forty-four compositions have the differences between Xanthii Herba and Xanthii Fructus. Forty-one chemical compositions are identified. Among of them, there are 12 kinds of differential compositions which presented different changing laws. Conclusion: From the different compositions of Xanthii Herba and Xanthii Fructus, the material basis can be provided for revealing the property and efficacy in Xanthii Herba and Xanthii Fructus.
