Seed quality test methods of Marsdenia tenacissima
10.7501/j.issn.0253-2670.2016.23.025
- Author:
Xue-Feng XIAO
1
Author Information
1. Institute of Chinese Medicinal Materials, Nanjing Agricultural University
- Publication Type:Journal Article
- Keywords:
Marsdenia tenacissima (Roxb) Wight et Arn.;
Quality test methods;
Seed;
Seed germination;
Seed testing protocol
- From:
Chinese Traditional and Herbal Drugs
2016;47(23):4264-4270
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the testing methods for seed quality of Marsdenia tenacissima, and provide basis for establishing seed quality standard of M. tenacissima. Methods: The seed quality of M. tenacissima from different producing areas was measured based on the International Seed Testing Protocol made up by ISTA and Rules for Agricultural Seed Testing issued by China. Results: The samples weight of M. tenacissima were at least 900 g for purity analysis and were at least 90 g for testing. The 1 000-seed weight was determined by 500-seed method, and the water content was carried out by higher temperature (133 ± 2)℃ for 6 h. After soaking in water for 24 h, M. tenacissima seeds were cultured in wet sand at 30℃ for 1-8 d under illumination for germination testing. Seed viability was tested by TTC method in 35℃ for 3 h. Conclusion: The seed testing methods for quality items of M. tenacissima have been initially established.
