Effect of sodium houttuyfonate on quorum sensing system of Pseudomonas aeruginosa
10.7501/j.issn.0253-2670.2016.24.016
- Author:
Fang LI
1
Author Information
1. Bozhou Product Quality Supervision and Testing Institute
- Publication Type:Journal Article
- Keywords:
LasA proteases;
Pseudomonas aeruginosa;
Pyocyanin;
Quorum sensing;
Sodium houttuyfonate
- From:
Chinese Traditional and Herbal Drugs
2016;47(24):4388-4394
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanism of sodium houttuyfonate (SH) against Pseudomonas aeruginosa (PA) targeting quorum sensing system. Methods: The microdilution method was adopted to determine the minimal inhibitory concentration (MIC) of SH and azithromycin (AZM) for PA; The effects of SH on LasA proteases activity and pyocyanin production were detected by UV spectrophotometry; The semi-quantitative PCR and qRT-PCR were used to detect the expression changes of quorum sensing regulatory genes (lasI, rhlI, lasR, rhlR, phzM, pqsA, pslA, lasA, lasB, and toxA). Results: The MIC of P. aeruginosa for SH and AZM was 512 μg/mL and 64 μg/mL, respectively; SH could significantly inhibit the activity of LasA proteases, and pyocyanin of bacteria; The morphology of P. aeruginosa was observed by agar plates; Furthermore, semi-quantitative PCR and qRT-PCR results indicated that the expression of lasI, rhlI, lasR, lasA, pslA, and phzM was significantly impact by SH. Conclusion: The results of present study suggest that SH possesses anti-quorum sensing properties and plays a role against infection by anti-quorum sensing activity.
