Study on HPLC fingerprint of Yinhuang Drop Pills
10.7501/j.issn.0253-2670.2016.24.013
- Author:
De-Xiong DAI
1
Author Information
1. Zhejiang Wecome Pharmaceutical Company Limited
- Publication Type:Journal Article
- Keywords:
3,4-O- dicaffeoylquinic acid;
3,5-O-dicaffeoylquinic acid;
4,5-O-dicaffeoylquinic acid;
Baicalin;
Chlorogenic acid;
Cryptochlorogenic acid;
Fingerprint;
HPLC;
Neochlorogenic acid;
Yinhuang Drop Pills
- From:
Chinese Traditional and Herbal Drugs
2016;47(24):4370-4373
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the specific chromatogram of Yinhuang Drop Pills (YDP) by High performance liquid chromatography. Methods: The chromatographic column Kromasil 100-5C18 (250 mm × 4.6 mm, 5 μm) was used, acetonitrile-0.4% phosphoric acid as mobile phase with gradient elution, and the detection wavelength was 372 nm. Through similarity evaluation software, the similarity of specific chromatogram of 10 batches YDP were calculated. Results: The HPLC specific chromatogram of YDP showed 7 common peaks, of which 6 peaks (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid) from Lonicerae Flos, 1 common peaks (baicalin) from Scutellariae Radix; By comparing with the reference substances, identified 7 components, respectively, the neochlorogenic acid (peak 1), chlorogenic acid (peak 2), cryptochlorogenic acid (peak 3), 3,4-O-dicaffeoylquinic acid (peak 4), 3,5-O-dicaffeoylquinic acid (peak 5), 4,5-O-dicaffeoylquinic acid (peak 6), baicalin (peak 7). By the methodology validation, verified that this method has good precision, repeatability and stability, 10 batches of sample similarity is greater than 0.9. Conclusion: This method was available for the quality control of YDP.
