Rapid identification of Astragali Radix from different origins by UPLC combined with chemometrics methods
10.7501/j.issn.0253-2670.2017.01.025
- Author:
Jing-Xia HE
1
Author Information
1. College of pharmacy Chengdu college of TCM
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
Cluster analysis;
Discriminant analysis;
Flavonoids;
Origins;
Principal component analysis;
UPLC
- From:
Chinese Traditional and Herbal Drugs
2017;48(1):179-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the contents of four flavonoids in Astragali Radix by UPLC-DAD method, and to rapidly identify their origins using chemometric method. Methods: UPLC method was used for determining calycosin-7-O-β-D-glycoside, ononin, calycosin, and formononetin in 20 batches of Astragali Radix from five provinces. Three methods, such as clustering analysis, principal component analysis, and discriminant analysis were compared for comprehensive quality evaluation of Astragali Radix from different places. Results: Origins of Astragali Radix were successfully divided into three categories by cluster analysis and principal component analysis, based on the contents of the tested flavonoids. Discriminant analysis could accurately distinguish some origins, such as the Inner Mongolia Autonomous Region and Sichuan and Jilin Provinces, while Shaanxi and Gansu Provinces appeared false classification. The correct rate of the initial group was 85%. Conclusion: UPLC combined with chemometric method could be used for rapid and accurate identification of origins of Astragali Radix, which also provided the scientific basis for the quality evaluation of Astragali Radix from different origins.
