Fast measurement method based on near-infrared spectroscopy in concentration of alcohol precipitation proee for Artemisiae Annuae Herba and Lonicerae Flos in Reduning Injection
10.7501/j.issn.0253-2670.2017.01.014
- Author:
Yong-Xiang WANG
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Chlorogenin acid;
Concentration of alcohol precipitation proee for Artemisiae Annuae Herba and Lonicerae Flos;
Cryptochlorogenin acid;
Near infrared spectroscopy technology;
Neochlorogenic acid;
Partial least squares;
Rapid quantitative analysis models;
Reduning Injection
- From:
Chinese Traditional and Herbal Drugs
2017;48(1):102-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The calibration models were developed in the concentration of alcohol precipitation proee for Artemisiae Annuae Herba (AAH) and Lonicerae Flos (LF) in Reduning Injection (RI) to realize the on-line monitoring of production process. Methods: Based on the near infrared reflectance spectroscopy (NIRS), partial least regression (PLS) models were developed to fast measure the contents of neochlorogenic acid, chlorogenic acid, and 4-O-caffeoylquinic acid in the concentration of the alcohol precipitation proee for AAH and LF. Results: In the quantitative models of neochlorogenic acid, chlorogenic acid, and 4-O-caffeoylquinic acid, the coefficient of determination (R2) of cross validation sets were 0.954 5, 0.975 2, and 0.969 1; The root mean square errors of calibration (RMSEC) were 0.213, 0.676, and 0.225; The root mean square errors of cross-validation (RMSECV) were 0.233, 0.692, and 0.258. When the established models were applied to on-line monitoring, the coefficient of determination of neochlorogenic acid, chlorogenic acid, and 4-O-caffeoylquinic acid were 0.984 2, 0.983 7, and 0.987 0, the residual predictive deviation (RPD) were 4.77, 5.29, and 4.37; The relative standard errors of prediction (RSEP) were 3.519%, 3.778%, and 3.895%. Conclusion: The models above are proved to fast measure the contents of neochlorogenic acid, chlorogenic acid, and 4-O-caffeoylquinic acid in the concentration of alcohol precipitation proee for AAH and LF in RI.
