Separation process of three phenolic glycosides from Monochasma savatieri
10.7501/j.issn.0253-2670.2016.02.012
- Author:
Yan-Kun CUI
1
Author Information
1. Heilongjiang University of Chinese Medicine, Heilongjiang Province
- Publication Type:Journal Article
- Keywords:
Acteoside;
Adsorption resins;
Dynamic axial column;
Isoacteoside;
Monochasmasavatieri Franch.;
Phenylethanoid glycosides;
Torenoside B
- From:
Chinese Traditional and Herbal Drugs
2017;48(2):288-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The optimal simultaneous separation process of three phenylethanoid glycosides (acteoside, isoacteoside, and torenoside B) from Monochasmasavatieri were established using of macrophage resins and dynamic axial column (DAC). Methods: The adsorption/desorption experiments and dynamic separation experiments were performed on eight types of resins (AB-8, D 101, HPD 100, LSA-10, LX-11, LX-17, LX-38, and XDA-6) to find the optimal resin. Then the optimal separation parameters were investigated on the chosen resin. The total phenylethanoid glycosides obtained from the large-scale experiment were further seperated to get acteoside, isoacteoside, and torenoside B, respectively using of DAC system. Results: Among these candidate resins, LX-17 was chosen to further obtain the optimal parameters: The optimal feeding concentration of raw materials was 1.8 g/mL; The optimal adsorption time was 150 min; The optimal gradient elute conditions were ethanol/water (0/100, 4 BV; 15/85, 4 BV; 60/40, 5 BV; 90/10, 2 BV). The large-scale experiments were amplified to 10 folds on the basis of optimal parameters to obtain total phenylethanoid glycosides. Acteoside, isoacteoside, and torenoside B were simultaneously obtained from total phenylethanoidglycosides using DAC system. Conclusion: LX-17 and DAC system can be used for the purification of phenylethanoid glycosides, which will have a goodfuture for the application in industry.
