Determination of curcumin, desmethoxycurumin, and bisdesmethoxycurum in Curcuma longa by QAMS
10.7501/j.issn.0253-2670.2017.03.024
- Author:
Wen-Bing LI
1
Author Information
1. Neo-Green Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Bisdesmethoxycurum;
Curcuma longa L.;
Curcumin;
Desmethoxycurumin;
QAMS;
Relative correlation factors
- From:
Chinese Traditional and Herbal Drugs
2017;48(3):573-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quality evaluation method for Curcuma longa, quantitative of multi-component with a single-marker (QAMS), to determine the contents of three curcuminoids at the same time. Methods: An HPLC method was used, and a Chromstar™ C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was methanol-4% glacial acetic acid (48:52) at a flow of 1.0 mL/min. The detection wavelength was 422 nm and column temperature was 30℃. Curcumin was used as the internal reference substance. The relative correlation factors of curcumin, desmethoxycurumin, and bisdesmethoxycurumin to curcumin were calculated and evaluated by standard curve method and QAMS. Results: The QAMS method could be used to determine the contents of three curcuminoids at the same time, and with no significant difference between the results of standard curve method (RSD < 2.0%). Conclusion: QAMS method is simple, feasible, and reproducible, which could provide a reference for further research on completing the quality control of raw material and processed C. longa.
