Simultaneous determination of 10 components in Yinhuang preparations by UPLC
10.7501/j.issn.0253-2670.2017.03.014
- Author:
Hou-Ning LONG
1
Author Information
1. College of Pharmacy, Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Bacicalin;
Baicalein;
Chlorogenic acid;
Cryptochlorogenic acid;
Isochlorogenic acid A;
Isochlorogenic acid B;
Isochlorogenic acid C;
Neochlorogenic acid;
Quality control;
UPLC;
Wogonin;
Wogonoside;
Yinhuang preparations
- From:
Chinese Traditional and Herbal Drugs
2017;48(3):505-510
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an UPLC method for simultaneous determination the contents of 10 active constituents [neochlorogenic acid (NCA), chlorogenic acid (CA), cryptochlorogenic acid (CCA), isochlorogenic acid A (ICAA), isochlorogenic acid B (ICAB), isochlorogenic acid C (ICAC); bacicalin, wogonoside, baicalein, and wogonin] in Yinhuang preparations. Methods: Analysis was performed on an Acquiyt UPLC BEH C18 chromatographic column (100 mm × 2.1 mm, 1.7 μm) eluted with acetonitrile- 0.4% phosphoric acid, gradient elution, and the flow rate was 0.4 mL/min, the detection wavelength was set at 326 nm, the column temperature was 40℃, and the injection volume was 1.0 μL. Results: All calibration curves were linear (r ≥ 0.999 6) over the tested ranges. The average recoveries (n = 9) ranged from 97.43% to 99.94% with RSD value below 2.0%. The contents of 11 batches of NCA, CA, CCA, ICAA, ICAB, ICAC, bacicalin, wogonoside, baicalein, and wogonin were 0.236-3.419, 5.279-26.220, 0.495-4.714, 0.130-2.702, 0.310-3.210, 0.363-5.036, 35.209-133.289, 1.493-6.635, 1.546-5.393, and 0.254-0.823 mg/g. Conclusion: This method is rapid, simple, sensitive, accurate, and reproducible, which can be used for quality control of Yinhuang preparation, and provide a reference for the formulation of quality standards to enhance in the future.
