Determination of five isoflavonoids in Pueraria Radix by QAMS
10.7501/j.issn.0253-2670.2017.04.026
- Author:
Wen-Jie WU
1
Author Information
1. School of Pharmacy, Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
6″-O-xylosidepuerarin;
Daidzein;
Daidzin;
Mirificin;
Pueraria Radix;
Puerarin;
QAMS;
Quality control;
Relative correction factor
- From:
Chinese Traditional and Herbal Drugs
2017;48(4):777-781
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for simultaneously determination of five isoflavones (daidzein, daidzin, puerarin, mirificin, and 6″-O-xylosidepuerarin) in Pueraria Radix by single marker (QAMS), which is feasible and accurate. Methods: Puerarin was taken as internal standard substance to establish the relative correction factor (RCF) for quantitative analysis of multi-components with QAMS. Thus, the contents of mirificin, 6″-O-xylosidepuerarin, daidzin, and daidzein were calculated. The contents in 10 batches of samples were determined by external standard method and QAMS. The scientificalness and feasibility of the methods were evaluated by comparison of the quantitative results between external standard method and QAMS. Results: The reproducibility of RCF was perfect. The results calculated with QAMS were consistent with the results by the external standard method. Conclusion: QAMS is accurate and feasible to evaluate the quality of Pueraria Radix.
