HPLC fingerprint identification of Lonicera Japonica Flos and Lonicera Flos
10.7501/j.issn.0253-2670.2017.04.025
- Author:
Ya-Nan LIU
1
Author Information
1. Henan Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
HPLC-fingerprint profiles;
Lonicera Flos;
Lonicera Japonica Flos;
Screening large peak integration;
Similarity evaluation
- From:
Chinese Traditional and Herbal Drugs
2017;48(4):773-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the differences between Lonicera Japonica Flos and Lonicera Flos by establishing HPLC fingerprint and calculating the similarity. Methods: The columns was Phenomenex Luna 5 μm C18 (2) 100 A, 250 mm×4.6 mm; The column temperature was 40℃. The mobile phase was acetonitrile-0.5% phosphoric acid, the flow rate was 1 mL/min, and the wavelength was 350 nm. Results: HPLC fingerprint of Lonicera Japonica and similarity evaluation by screening large peak integration were established. The similarity of 12 batches of Lonicera Japonica Flos were all above 0.95, and four batches of Lonicera Flos were less than 0.80. Conclusion: HPLC fingerprint profiles under 350 nm can reflex the differences between Lonicera Japonica Flos and Lonicera Flos effectively; Similarity evaluation by screening large peak integration shows the tiny differences of chemical component.
