Chemical constituents and activity of stem extract from Orostachys malacophyllus in Changbai Mountain
10.7501/j.issn.0253-2670.2017.05.003
- Author:
Xiu-Mei YIN
1
Author Information
1. College of Pharmacy, Yanbian University
- Publication Type:Journal Article
- Keywords:
1-ethyl-2-hydroxysuccinate;
1-ethyl-2-O-[(3,4-dihydroxybenzoyl) oxy]-succinate;
Cytotoxicity;
Diisobutyl phthalate;
Orostachys malacophyllus (Pall.) Fisch.;
Orostachysolic acid
- From:
Chinese Traditional and Herbal Drugs
2017;48(5):859-862
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the active constituents from the stems of Orostachys malacophyllus in Changbai Mountain. Methods: The ethyl acetate fraction of the methanol extract from the stems of O. malacophyllus was separated by chromatographic methods, structure identification was by means of nuclear magnetic resonance and mass spectra, and the activity was determined by CCK-8 experiment. Results: Five compounds were isolated and identified as 1-ethyl-2-hydroxysuccinate (1), 1-ethyl-2-O-[(3,4-dihydroxybenzoyl) oxy]-succinate (2), 1,4-dimethy-2-O-[(3,4-dihydroxybenzoyl) oxy]-succinate (3), 1,4-dimethy-2-O-[(4-dihydroxybenzoyl) oxy]-succinate (4), and diisobutyl phthalate (5). Conclusion: Compounds 1-5 are isolated from the plants of this genus for the first time. Among them, compound 2 is a new compound named orostachysolic acid, and exhibits cytotoxicity against HeLa cell with IC50 value of 111.5 μmol/L.
