Apoptotic effect of arundoin on human prostatic cancer cells by activating Poly ADP ribose polymerase
10.7501/j.issn.0253-2670.2017.06.021
- Author:
Da-Ke CHEN
1
Author Information
1. Urinary Surgery Department, Wenzhou Municipal People's Hospital
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Arundoin;
Imperata cylindrica Beauv. var. major (Nees) C. E. Hubb.;
Poly ADP;
Prostate cancer;
ribose polymerase;
Triterpenoid
- From:
Chinese Traditional and Herbal Drugs
2017;48(6):1183-1187
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the potential apoptotic effect of arundoin on human prostatic cancer cells. Methods: Arundoin was isolated and purified from the crude ethanol extract of Imperatae Rhizoma. Human prostatic cancer cell line PC3 was treated with arundoin. The relative cell viabilities were determined by MTT assay; Apoptosis was analyzed by Annexin V/PI dual staining with flow cytometry; The protein expression levels of Poly ADP ribose polymerase (PARP) and other apoptotic related proteins were detected by Western blotting. Results: Arundoin (20, 40, and 80 μmol/L) reduced the viability of PC3 cells dose- and time-dependently. Moreover, arundoin induced apoptosis in PC3 cells. Western blotting analysis showed that arundoin up-regulated the expression level of PARP in a dose-dependent manner. In addition, apoptosis related proteins such as Bax, Bcl-2, and casapases were all affected by arundoin. Conclusion: Arundoin could induce apoptosis in human prostatic cancer cells, which provides novel information for clinical application of arundoin and its preparations for the prostatic cancer patients.
