Cloning and mRNA expression analysis of phosphatidylinositol-3,4,5- trisphosphate 3-phosphatase gene from Tripterygium wilfordii
10.7501/j.issn.0253-2670.2017.07.022
- Author:
Jia-Wei ZHOU
1
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University
- Publication Type:Journal Article
- Keywords:
Bioinformatics analysis;
Clone;
Expression analysis;
PTEN;
Tripterygium wilfordii Hook. f.
- From:
Chinese Traditional and Herbal Drugs
2017;48(7):1391-1396
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone the full-length cDNA of phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase in Tripterygium wilfordii (TwPTEN) and predicted its biological functions. Methods: The specific primers were designed based on the transcriptome data of Tripterygium wilfordii, TwPTEN gene was cloned by Polymerase Chain Reaction. The full-length cDNA of TwPTEN was then analyzed by a series of bioinformatics analysis. Results: It was showed that the full length of TwPTEN cDNA was 2 247 bp encoding 614 amino acids. The theoretical isoelectric point was 5.84 and the molecular weight was about 66 900. Sequence alignment and phylogenetic analysis demonstrated that TwPTEN had relative close relationship to PTEN from other species. Differential expression analysis revealed that the relative expression level of TwPTEN increased significantly after being induced by methyl jasmonate (MeJA). It indicated that the TwPTEN gene was relative to the biosynthesis of secondary metabolites. Conclusion: PTEN gene is firstly cloned from T. wilfordii, which provides a basis for further studying the functions of TwPTEN.
