Preparation and quality evaluation of silybin proliposomes
10.7501/j.issn.0253-2670.2017.07.009
- Author:
Xiao-Ning WANG
1
Author Information
1. College of Pharmacy, Xi'an Medical University
- Publication Type:Journal Article
- Keywords:
Cryoprotectant;
Drug loading dosage;
Encapsulation efficiency;
Film dispersion method;
Freeze-drying method;
Orthogonal design;
Proliposomes;
Quality evaluation;
Silybin;
Single factor investigation;
Stability
- From:
Chinese Traditional and Herbal Drugs
2017;48(7):1314-1320
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare silybin (SLB) proliposomes and evaluate its quality. Methods: SLB proliposomes were prepared by freeze-drying method, and the formulation and process were optimized by single factor investigation and orthogonal design with the encapsulation efficiency and drug loading as indexes. The optimal cryoprotetant was selected and the morphology, particle size, encapsulation efficiency, and stability of SLB proliposomes were investigated. Results: The optimized preparation process was as follows: The ratio of drug to total lipid was 1:12, the ratio of phospholipid to cholesterol was 4:1, the pH of hydration medium was 7.4 and the temperature was 45℃. Mannitol was the optimal cryprotectant to prepare SLB proliposomes, and the formation of SLB proliposomes looked plumpy and compact. The size of preliposome was around (251.40 ± 2.14) nm, the Zeta potential was around (-30.80 ± 0.89) mV, encapsulation efficiency was (88.92 ± 5.86)%, and it had good stability during storage. Conclusion: The preparation process of SLB proliposomes is simple, and it has high encapsulation efficiency and good stability, therefore, it is deserved to be further studied.
