Simultaneous determination of eight flavone glycosides in total flavonoids fraction of Microcos paniculata using quantitative analysis of multi-components by single marker method
10.7501/j.issn.0253-2670.2017.09.027
- Author:
Chang-Le LIU
1
Author Information
1. School of Pharmacy, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Apigenin-6, 8-di-C-β-D-glucopyranoside;
Apigenin-6-C-α-L-arabino-pyranosyl-8-C-β-D-glucopyranoside;
Apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside;
Apigenin-6-C-β-D-xylopyranosyl-8-C-β-D-glucopyranoside;
HPLC;
Isorhamnetin-3-O-β-D-rutinoside;
Isovitexin;
Kaempferol-3-O-β-D-glucopyranoside;
Microcos paniculata L.;
QAMS;
Relative correction factors;
Vitexin
- From:
Chinese Traditional and Herbal Drugs
2017;48(9):1872-1877
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for simultaneous determination of eight flavone glycosides in total flavonoids fraction of Microcos panicμLata. Methods: A new method, quantitative analysis of multi-components by single marker (QAMS), was established using vitexin as reference substance. The corresponding relative correction factors of apigenin-6, 8-di-C-β-D-glucopyranoside, apigenin-6-C-α-L-arabino-pyranosyl-8-C-β-D-glucopyranoside, apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside, apigenin- 6-C-β-D-xylopyranosyl-8-C-β-D-glucopyranoside, isovitexin, kaempferol-3-O-β-D-glucopyranoside, and isorhamnetin-3- O-β-D-rutinoside compared with vitexin were calculated. Then, the contents of eight flavonoid glycosides were determined. Results: The relative retention time and relative correction factors of the eight flavone glycosides were repeatedly and the relative correction factors of the other seven flavonoid glycosides were 1.01, 0.60, 0.76, 2.02, 1.05, 1.14, and 2.42, respectively. There were no significant differences in the results between ESM and QAMS method for 10 batches of extract. Conclusion; The established QAMS method for simultaneous determination of eight flavone glycosides is simple and robust, and can be used for related research and quality control of the total flavonoids fraction of M. panicμLata.
