Mechanism of Coriaria sinica extract on anti-methicillin resistant Staphylococcus aureus
10.7501/j.issn.0253-2670.2017.09.016
- Author:
De-Bin HUANG
1
Author Information
1. Department of Pharmacy, Hubei University for Nationalities
- Publication Type:Journal Article
- Keywords:
Coriaria sinica extract (CSE);
Methicillin resistant Staphylococcus aureus (MRSA);
PBPs;
Synergies mechanism;
β-lactam antibiotics
- From:
Chinese Traditional and Herbal Drugs
2017;48(9):1802-1811
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the synergies mechanism of Coriaria sinica extract (CSE) combined with β-lactam antibiotics on methicillin resistant Staphylococcus aureus (MRSA). Methods: The relevant gene expression, autolytic enzyme, and influence of β-lactamase were determined by AFFX prokaryotic expression microarray, Western-blotting, SDS-PAGE gel electrophoresis, etc. Results: MRSA was resistant to the most antibiotics, and it had significant synergistic antibacterial effect while CSE was combined with β-lactam antibiotics (P < 0.05). The CSE can significantly reduce the total expression of RNA and regulate the expression of many genes with showing a dose-dependence when used alone or combined with ampicillin (AP), such as the basal metabolism genes, peptidoglycan hydrolase gene (lytM), transporter gene, PBPs, β-lactamase activity, etc. (P < 0.05). It can significantly improve the concentration of cefotaxime (CFX) in internal of MRSA (P < 0.05). Conclusion: The CSE has significant inhibitory effects on MRSA, and it has significant synergistic effects when combined with β-lactam antibiotic on MRSA. The mechanism is associated with many factors of MRSA, such as regulation of expression and transcription on target genes (ribA, PBPs, lytM, etc.), the influence of active efflux, autolysis and metabolism, etc.
