Determination of eight constituents in Anemarrhenae Rhizoma before and after salt processing by HPLC-MS
10.7501/j.issn.0253-2670.2017.09.014
- Author:
De JI
1
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Anemarrhenae Rhizoma;
Anemarrhenasaponin I;
HPLC-MS;
Mangiferin;
Neomangiferin;
Salt processing;
Timosaponin AII;
Timosaponin AIII;
Timosaponin BII;
Timosaponin BIII;
Timosaponin E1
- From:
Chinese Traditional and Herbal Drugs
2017;48(9):1784-1790
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC-MS method for the simultaneous quantitation of neomangiferin, mangiferin, timosaponin E1, timosaponin BII, timosaponin BIII, anemarrhenasaponin I, timosaponin AIII, and timosaponin AII in Anemarrhenae Rhizoma, and to analyze the content change before and after salt processing. Methods: Chromatography was performed on Agilent SB-C18 (50 mm × 2.1 mm, 1.8 μm) column by gradient elution using 0.05% formic acid in water (A)-acetonitrile (B) at the flow rate of 0.4 mL/min. The column temperature was kept at 30 ℃ and the inject volume was 10 μL. HPLC-MS was used for quantification in negative ion mode under ESI. Results: The eight components had good linearity (r ≥ 0.9997) within the linear range. The average recovery rate was 97.55%-103.01% with RSD < 2.80%. The contents of the eight components in Anemarrhenae Rhizoma varied according to the different habits. The contents of mangiferin, timosaponin BII, and timosaponin AIII were high. After salt processing, contents of timosaponin E1, timosaponin BII, and anemarrhenasaponin I were reduced, content of timosaponin BIII was increased and contents of neomangiferin, mangiferin, timosaponin AIII, and timosaponin AII had no obvious changes. Conclusion: The changes of components in Anemarrhenae Rhizoma before and after salt processing are proved by the method of HPLC-MS which is simple, sensitive, and accurate.
