Simultaneous determination of four constituents in Zizyphi Spinosae Semen by ASE-CAD
10.7501/j.issn.0253-2670.2017.10.012
- Author:
Xue-Song CHEN
1
Author Information
1. Wuzhou Institute for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
Accelerated solvent extraction;
Betulinic acid;
Charged aerosol detector;
Jujuboside A;
Jujuboside B;
Spinosin;
Zizyphi Spinosae Semen
- From:
Chinese Traditional and Herbal Drugs
2017;48(10):2002-2006
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an accelerated solvent extraction (ASE)-charged aerosol detector (CAD) method for simultaneous detection of jujuboside A, jujuboside B, spinosin, and betulinic acid in Zizyphi Spinosae Semen. Methods: The orthogonal design was applied to optimize the extraction parameters of the ASE system. The target compounds were detected by HPLC-CAD with the parameters as follow: Thermo Syncronis C18 (100 mm × 3 mm, 3 μm) column, a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 0.5 mL/min, the column temperature was kept at 40℃. Detector was Corona Ultra CAD with 35℃ of nebulization temperature. Results: Optimization of the ASE parameters with orthogonal design greatly improved the extraction efficiency; All the target compounds could be simultaneously determined in a single run. Good linear relationships (0.998 3-0.999 6) and high relative recoveries were 98.46%-102.02%. Conclusion: The method is rapid, simple, accurate, and thus could be used for the quality control of Zizyphi Spinosae Semen.
