Effects of tannins from Granati Pericarpium on endogenous substance metabolism and analysis on metabolic pathway in rats with glomeruloscerosis
10.7501/j.issn.0253-2670.2017.10.023
- Author:
Ben-Hong ZHOU
1
Author Information
1. Department of Pharmacy, Renmin Hospital of Wuhan University
- Publication Type:Journal Article
- Keywords:
Alanine;
Aspartate;
Biomarker;
Glomeruloscerosis;
Glutamate;
Tannins from Punica granatum;
Tryptophan;
Urinary metabolomics
- From:
Chinese Traditional and Herbal Drugs
2017;48(10):2072-2080
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A urinary metabonomics method based on liquid chromatography-mass spectrometry (HPLC-MS) was used to inspect the effect of tannins from Granati Pericarpium on endogenous metabolism of rats with glomeruloscerosis, the potential biomarkers were searched and the metabolic pathways were analyzed to provide relevant theory for treatment of glomeruloscerosis. Methods: Forty-eight SD rats were divided into control group, model group, benazepril hydrochloridec group, and three doses of pomerganate tannis groups. Glomerular sclerosis model was established by unilateral nephrectomy combined with two tail iv adriamycin; 24 h urine of rats was collected after the rats were orally given tannins from Granati Pericarpium for 8 weeks; HPLC-MS was used for testing and analyzing the urine; Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used for data treatment of each group to search the potential biomarkers and build the metabolicomics pathways. Results: The urinary metabolism of rats was clustering. Ten potential biomakers were screened by analysis and identification of important variables; The metabonomics pathways involved tryptophan, alanine, aspartate, glutamate metabolism, etc. Conclusion: The trend of endogenous metabolism of rats with glomeruloscerosis is normal after intervened by tannis in pomegranate tannins from Granati Pericarpium. It provides the basement with the further research of clarifying the mechanism of tannins from Granati Pericarpium inpacting on rats with glomeruloscerosis.
