Rapid determination of oleanolic acid and ursolic acid in eight kinds of Chinese materia medica by HPLC
10.7501/j.issn.0253-2670.2017.10.011
- Author:
Hui-Yan LIU
1
Author Information
1. Wu Zhou Institutes for Food and Drug Control
- Publication Type:Journal Article
- Keywords:
Accelerated solvent extraction;
Chaenomeles Fructus papaya;
Crataegus pinnatifida;
Eriobotrya japonica;
Hedyotis diffusa;
HPLC;
Ligustrum lucidum;
Oleanolic acid;
Patrinia scabiosaefolia;
Prunella vulgaris;
Ursolic acid;
Verbena officinalis
- From:
Chinese Traditional and Herbal Drugs
2017;48(10):1998-2001
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method of rapid analysis for simultaneous determination of oleanolic acid and ursolic acid in Chinese materia medica (CMM) of Verbena officinalis, Ligustrum lucidum, Prunella vulgaris, Hedyotis diffusa, Patrinia scabiosaefolia, Eriobotrya japonica, Crataegus pinnatifida, Chaenomeles Fructus papaya. Methods: The analyses of preparation were conducted by accelerated solvent extraction (ASE), methanol was used as solvent extraction, and the static extraction time was 6 min. Separation was carried out on Acclaim C30 Thermo column with acetonitrile and 0.2% acetic acid (85:15) as mobile phase, flow rate was 0.3 mL/min, and UV detection wavelength was set at 205 nm. Results: After 10 min sample extraction time, oleanolic acid and ursolic acid reached baseline separation, and no sample matrix was interfered, the linear correlation coefficient was over 0.999, the average recovery between 95.8% and 102.7%; Compared to Chinese Pharmacopoeia 2015, the determination of average mass fraction difference was 3.7% and 5.8%. Conclusion: This method is simple, fast, and suitable for the analysis of these drugs, and it can be used for content determination of oleanolic acid and ursolic acid for eight kinds of CMM.
