Bioassay-guided separation of effective components from Gentianella acuta and their protective effect on oxidative damage in PC12 cells induced by H2O2
10.7501/j.issn.0253-2670.2017.10.005
- Author:
Li-Juan LV
1
Author Information
1. Department of Basic Science, Tianjin Agricultural University
- Publication Type:Journal Article
- Keywords:
Bellidifolin;
Demethylbellidifolin;
Gentianella acuta (Michx.) Hulten;
H2O2;
Norswertianolin;
Oxidative stress;
PC12 cells;
Real-time cell analysis;
Swertianolin
- From:
Chinese Traditional and Herbal Drugs
2017;48(10):1957-1963
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the compounds of Gentianella acuta with strong protective effect on oxidative damage in PC12 cells induced by H2O2. Methods: Damage model of PC12 cells was established by H2O2 and real-time cell analysis (RTCA) was utilized to evaluate the protective effect of extracts and compounds. Meanwhile, bioassay-guided method was applied to separating effective components, and HPLC was used for the determination and structure confirmation of compounds. Results: Fractions of n-butanol and acetic ether showed protective effect on oxidative damage in PC12 cells induced by H2O2. The anti-oxidant activity of compounds 1 and 2 showed dose-dependent manner in the scope of 3.125-50.000 μmol/L, and the protective effect was the strongest at the concentration of 50.000 μmol/L. Compounds 3 and 5 showed the best protective effect at the concentration of 25.000 μmol/L and 3.125 μmol/L respectively. Compounds 1, 2, 3, and 5 were identified as bellidifolin, demethylbellidifolin, swertianolin and norswertianolin, respectively. Conclusion: Compounds 1, 2, 3, and 5 show protective effect on oxidative damage in PC12 cells induced by H2O2, maybe the main active components in G. acuta. But the mechanism is still uncertain, and further exploration is imperative.
