Simultaneous determination of 20 active constituents in Maiweishen by HPLC
10.7501/j.issn.0253-2670.2017.12.026
- Author:
Yu-Gang GAO
1
Author Information
1. College of Traditional Chinese Medicine, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Compound K;
Ginsenoside F1;
Ginsenoside F2;
Ginsenoside Rb1;
Ginsenoside Rb2;
Ginsenoside Rb3;
Ginsenoside Rc;
Ginsenoside Rd;
Ginsenoside Re;
Ginsenoside Rf;
Ginsenoside Rg2;
Ginsenoside Rg3;
Ginsenoside Rh2;
GinsenosideRg1;
HPLC;
Maiweishen;
Ophiopogonin D;
Protopanaxadiol;
Protopanaxatriol;
Schizandrin A;
Schizandrin B;
Schizandrol A
- From:
Chinese Traditional and Herbal Drugs
2017;48(12):2527-2531
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the quality of Maiweishen, a simple and accurate HPLC method for determining the contents of 20 active constituents from Maiweishen was established. Methods: The chromatographic separation was achieved on a C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase made up of acetonitrile and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35 ℃, respectively. Results: Sixteen ginsenosides (Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, compounds K, Rh2, and protopanaxadiol), three kinds of lignan in Schisandra chinensis (schizandrol A, schizandrin A, B), and ophiopogonin D were separated at baseline with good linearity (r ≥ 0.999 6). The recovery rates were 96%-102% (RSD < 2%). Conclusion: The method is simple, fast, accurate, and could be applied to the quality control of Maiweishen.
