Effects of total flavonoids of Actinidiae Radix on anti-oxidant capacity and related gene expression in rat liver tissues
10.7501/j.issn.0253-2670.2017.12.019
- Author:
Kun TENG
1
Author Information
1. College of Pharmaceutical and Food Science, Tonghua Normal University
- Publication Type:Journal Article
- Keywords:
Actinidiae Radix;
Anti-oxidant;
CAT;
MDA;
SOD;
Total flavonoids
- From:
Chinese Traditional and Herbal Drugs
2017;48(12):2486-2491
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the antioxidant activities of total flavonoids of Actinidiae Radix (TFAR) in vitro and in vivo and the related mechanism. Methods: The anti-oxidant activities of TFAR were determined using DPPH and ABTS radical scavenging assays in vitro. The content of MDA and the activity of SOD and CAT in rat liver tissue were determined. Results: TFAR have showed significant DPPH radical scavenging activity and ABTS free radical scavenging activity and the IC50 values of TFAR were 27.01 and 29.55 mg/mL, respectively. Compared with the model group, the MDA content of liver tissue was significantly decreased in the order of TFAR from low dose to high dose, and the activity of SOD and CAT increased significantly. Compared with model group, gene expression of mRNA of SOD1, SOD2 and GPX1 in liver tissue was up-regulated by each dose group of TFAR. Conclusion: TFAR has anti-oxidant activity in vitro and in vivo, and has a dose-dependent relationship with the expression of mRNA in rat liver tissue.
