Anticancer effects and mechanism of methyl haematommate
10.7501/j.issn.0253-2670.2017.12.017
- Author:
Jing-Lin SHUI
1
Author Information
1. School of Public Health, Kunming Medical University
- Publication Type:Journal Article
- Keywords:
Antitumor activity;
Cell cycle;
Methyl haematommate;
Microarray;
Usnea diffracta Vain.
- From:
Chinese Traditional and Herbal Drugs
2017;48(12):2474-2480
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the in vitro inhibitory effect of methyl haematommate (a new bioactive compound in Usnea), on the Xuanwei lung cancer cell line (XWLC-05), Hepato carcinoma cell line (HepG2), and breast carcinoma cell line (MCF-7), and investigate its mechanism. Methods: MTT assay was used to determine the inhibitory effect of methyl haematommate on the three cancer cell lines at different concentration (2, 4, 8, 16, and 32 μg/mL). Cell cycle of HepG2 was analyzed by flow cytometry (FCM) and microarray assay was used to identify the differentially expressed gene profiles in MCF-7. Results: MTT results showed that methyl haematommate could significantly inhibit the proliferation of cancer cells, and the inhibition was concentration-dependent. IC50 values of the compound were 8.818, 11.905, and 13.328 μg/mL in XWLC, HepG2, and MCF-7 respectively. Cell cycle analysis indicated that methyl haematommate could arrest cancer cells at G0/G1. Totally 2 394 mRNAs were significantly regulated by the compound in MCF-7 (fold change ≥ 1.5, P < 0.05), of which 789 were up-regulated and 1 605 were down-regulated. Conclusion: Methyl haematommate is isolated from Usnea diffracta for the first time, and it shows inhibitory effects on hunman cancer cell lines in vitro. MAPK pathway and G0/G1 arrest might contribute to the anticancer effects of methyl hematommate.
