Study on process of extraction and purification of water-soluble alkaloids from Aconiti Lateralis Radix Praeparata
10.7501/j.issn.0253-2670.2017.12.009
- Author:
Chun-Mei LUO
1
Author Information
1. School of Pharmacy, Southwest Medical University
- Publication Type:Journal Article
- Keywords:
Aconine;
Aconiti Lateralis Radix Praeparata;
Aconitine;
Benzoylaconine;
Benzoylhypaconine;
Benzoylmeaconine;
Fuziline;
Hypaconine;
Hypaconitine;
Karacoline;
Lannaconitine;
Macroporous resin;
Mesaconine;
Mesaconitine;
Neoline;
Songorine;
Talatisamine;
UPLC-MS/MS;
Water-soluble alkaloids
- From:
Chinese Traditional and Herbal Drugs
2017;48(12):2415-2424
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the process of extraction and purification of water-soluble alkaloids of Aconiti Lateralis Radix Praeparata (ALRP). Methods: Orthogonal design L9(34) and single factor experiments were employed to optimize the extraction conditions using UPLC-MS/MS. The total extraction yield of 14 alkaloids (karacoline, songorine, fuziline, neoline, talatisamine, mesaconine, aconine, hypaconine, benzoylmeaconine, benzoylaconine, benzoylhypaconine, mesaconitine, aconitine, and hypaconitine) was used as an index. The absorption-desorption characteristics of five macroporous resins were evaluated to optimize purification conditions using contents of eight water-soluble alkaloids (aconine, mesaconine, hypaconine, fuziline, neoline, talatisamine, songorine, and karacoline) as indexes. Results: The optimized extraction and purification conditions were as follows: steamed tablets were decocted thrice with 10-fold pH 3.5 acidic water for 2 h each time (maintaining pH 3.5). The alkalinity of water decoction was adjusted to pH 10.0 with 20% NaOH and then heated for 2 h. The supernatant was adjusted to pH 11.0 by 20% NaOH and then was subjected to macroporous resin HPD300 whose relative adsorption amount was 2.5 g dried medicinal herb/mL resin, and then the resin was eluted with 6 BV of water and 4 BV of 80% ethanol respectively. The 80% ethanol fraction was evaporated under vacuum to give the water-soluble alkaloids extract. The extract yield was 1.69%. The total contents of mesaconine, aconine, hypaconine, fuziline, neoline, talatisamine, karacoline and songorine were above 15%. Conclusion: The optimized extraction and purification process is stable and feasible, and this present study provides the reference for the research and application of water-soluble alkaloids in ALRP.
