Inhibition of dihydrotanshinone on migration and invasion of gastric cancer SGC7901 cells and its mechanism
10.7501/j.issn.0253-2670.2017.15.020
- Author:
Yu-Qing GE
1
Author Information
1. The First Affiliated Hospital, Zhejiang Chinese Medical University
- Publication Type:Journal Article
- Keywords:
Dihydrotanshinone;
Gastric cancer;
Hedgehog pathway;
Matrix metalloproteinases;
Migration and invasion
- From:
Chinese Traditional and Herbal Drugs
2017;48(15):3138-3144
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of dihydrotanshinone (DHT) on the migration and invasion of human gastric cancer SGC7901 cells and to investigate its mechanism. Methods: SGC7901 cells were treated with different concentration of DHT. Then, the inhibitory effect of DHT was detected by MTT assay. The scratch adhesion test and Transwell assay were performed to determine the migration and invasion capacity of the cells. Quantitative PCR and Western boltting were used to examine the expression of MMP2, MMP9, Gli1, and HHIP in SGC7901 cells. Results: DHT could inhibit the proliferation of SGC7901 cells with obvious dose- and time- dependent effects. DHT significantly inhibited the migration and invasion ability in SGC7901 cells in vitro. The expression of MMP9 was obviously down-regulated after DHT treatment. Furthermore, DHT significantly inhibited the Gli1 mRNA and proteins expression levels, and evaluated the expression of HHIP in SGC7901 cells. Conclusion: DHT could inhibit the capability of migration and invasion of human gastric cancer SGC7901 cells. The potential molecular mechanism may be related to the inhibition of MMP9, and down-regulation of Hedgehog signaling pathway.
