Evaluation of Toosendan Fructus-induced hepatotoxicity based on metabolic profile of fatty acids
10.7501/j.issn.0253-2670.2017.15.014
- Author:
Yin-Hua XIONG
1
Author Information
1. School of Pharmacy, Jiangxi Science and Technology Normal University
- Publication Type:Journal Article
- Keywords:
Arachidonic acid;
Corydalis Rhizoma;
Fatty acids;
Hepatotoxicity;
Metabolic profile;
Palmitoleic acid;
Toosendan Fructus;
Vaccenic acid
- From:
Chinese Traditional and Herbal Drugs
2017;48(15):3104-3109
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the hepatotoxicity caused by water extract with alcohol precipitating of Toosendan Fructus (TF) and Toosendan Fructus + Corydalis Rhizoma (TF + CR) based on metabolic profiling of fatty acids in mice serum. Methods: A gas chromatography-mass spectrometry method was applied for simultaneous quantification of 15 fatty acids, including both non-esterified and esterified fatty acids, in the serum of control, TF-treated, and TF + CR-treated mice. Meanwhile, the change of fatty acid metabolic profile in liver injured mice was analyzed by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). Results: The result of PCA showed that the metabolic profile of serum fatty acids in TF-treated mice significantly deviated from the normal level, and CR with hepatoprotective effect could obviously reverse the deviation. More importantly, the result of PLS-DA illustrated that palmitoleic acid, vaccenic acid, and arachidonic acid had important contribution on the hepatotoxicity induced by TF. Therefore, the three fatty acids were identified as potential biomarkers. Conclusion: Hepatotoxicity caused by TF has a good correlation with the metabolic profiling of fatty acid. The project can provide foundation for further investigation on the evaluation and mechanism of TF-induced hepatotoxicity.
