Purification of total flavonoids extracted from Astragali Complanati Semen using D-101 macroporous adsorption resin
10.7501/j.issn.0253-2670.2017.16.014
- Author:
Ru-Ying TANG
1
Author Information
1. Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astragali Companati Semen;
Complanatoside A;
Extraction rate;
Macroporous adsorption resin;
Orthogonal experiment design;
Separation and purification;
Total flavonoids;
Transferation rate
- From:
Chinese Traditional and Herbal Drugs
2017;48(16):3342-3346
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the purification technology of total flavonoids from Astragali Companati Semen (ACS) by macroporous adsorption resin. Methods: The process parameters of water decoction extraction of ACS and purification of total flavonoids extracted from ACS by D-101 macroporous adsorption resin were optimized by orthogonal experiment design method. Results: The optimal extraction process were as follows: water decoction extract for three times, each time 0.5 h, water addition of 10, 6, and 6 times amount of the medicine respectively. The optimized technology conditions of D-101 macroporous adsorption resin were as follows: The flavonoids concentration of sample liquid of ACS was about 1.0 mg/mL, the diameter ratio was 1∶5, the sample flow rate was 1 BV/h, the rate of sample weight was 0.6 g/mL (herbs/resin); The velocity of water elution was 1 BV/h and its elution volume was 4 BV; The elution concentration, elution velocity, and elution volume were 70%, 1 BV/h, and 5 BV, respectively. The content paste rate of made ACS of total flavonoids was 2.65%, the mass fraction of total flavonoids was 56.24%, and the process transfer rate was 68.37%. Conclusion: The method is simple and feasible for purification of total flavonoids extracted from ACS.
