Study on UPLC-PDA fingerprint of Xueshuan Xinmaining Tablets
10.7501/j.issn.0253-2670.2017.20.022
- Author:
Hui-Jun HU
1
Author Information
1. Jiangxi University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Bufonis Venenum;
Chuanxiong Rhizoma;
Cinobufagin;
Cluster analysis;
Ferulic acid;
Fingerprint;
Ilicis Pubescentis Radix;
Medicinal materials of belonging;
Resibufogenin;
Rutin;
Salviae Miltiorrhizae Radix et Rhizoma;
Salvianolic acid B;
Similarity analysis;
Sophora Flos;
Tanshinone IIA;
UPLC-PDA;
Xueshuan Xinmaining Tablets
- From:
Chinese Traditional and Herbal Drugs
2017;48(20):4268-4274
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the consistency of 30 batches of Xueshuan Xinmaining Tablets (XXT), we establish the UPLC-PDA fingerprint of XXT and provide reliable scientific basis for the Chinese medicine quality consistency. Methods Methanol was used to extract the XXT samples, and the extracts were measured by UPLC with the absorption wavelength at 280 nm. Waters Acquity UPLC HSS T3 reversed-phase column (50 mm × 2.1 mm, 1.8 μm) was used for determining the extracts at a flow rate of 0.5 mL/min and column temperature of 40 ℃. The mobile phase condition was acetonitrile-0.1% formic acid aqueous solution with gradient mode. The methodology, similarity calculation and cluster analysis of 30 batches of XXT samples were investigated. The common peak of XXT was belonged to single medicinal materials and common peaks were identified by comparing with the reference. Results The UPLC fingerprint of XXT was established. Twenty-seven common fingerprint peaks were indicated with wavelength 280 nm, Among them, peaks 1, 10, 12-16, 18, and 21-28 belonged to Salviae Miltiorrhizae Radix et Rhizoma (SMRR), peaks 2-4, 6, 8, 9, and 17 belonged to Chuanxiong Rhizoma (CR), peaks 3, 7, 9, 11, and 16 belonged to Ilicis Pubescentis Radix (IPR), peak 5 belonged to Sophora Flos (SF), peaks 19 and 20 belonged to Bufonis Venenum (BV). Peaks 4, 5, 13, 19, 20, and 28 were ferulic acid, rutin, salvianolic acid B,resibufogenin, cinobufagin, tanshinone IIA, respectively, compared with reference. The analyzed samples were classified into two classes and the similarity was greater than 0.960. Conclusion The method of XXT UPLC-PDA fingerprint was established with good precision, stability and repeatability, supplying helpful information for the comprehensive evaluation of XXT.
