De novo assembly and transcriptome characterization of Penthorum chinense
10.7501/j.issn.0253-2670.2017.21.023
- Author:
Can YUAN
1
Author Information
1. Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences
- Publication Type:Journal Article
- Keywords:
Flavonoids;
Penthorum chinense Pursh.;
Quercetin;
Sterols;
Terpenoids;
Transcriptome
- From:
Chinese Traditional and Herbal Drugs
2017;48(21):4507-4514
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to obtain the reference sequences and relative expression of transcription genes and study the genetic base of active ingredients in Penthorum chinense, which were useful for researching functional gene to P. chinense. Methods In this study, by performing Illumina Hiseq 2000 and de novo assembly, the transcriptome of whole plant was sequenced, the data were filtered and assembled, and the unigene was compared and annotated. Meanwhile, the genes related to the synthesis of metabolic pathway of active ingredients in P. chinense were analyzed. Results Totally, 40 005 442 valid short sequences were obtained, and 42 306 unigenes were spliced by de novo. Also, a total of 518 open reading frames (ORF) were obtained by ORF analysis, and 75 ORF of them had transcription factor domains. In addition, by performing KEGG pathway analysis, 33, 32, 59, and 68 unigenes were found to be involved in the pathway of flavonoid biosynthesis, steroid biosynthesis, terpenoid backbone biosynthesis, and 2-oxocarboxylic acid metabolism, respectively. Conclusion The datasets provided in this study will contribute significantly to genetic improvement and study on the genes related to biosynthesis pathway of pharmaceutical active substances from P. chinense.
