Study on chemical constituents and dose-effect correlation of anti-oxidative activities among extracts of stems and leaves of Salvia miltiorrhiza
10.7501/j.issn.0253-2670.2017.22.015
- Author:
Hui-Ting ZENG
1
Author Information
1. Key Laboratory of Chinese Medicinal Resources Recycling Utilization, State Administration of Traditional Chinese Medicine, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Anti-oxidant activity;
Caffeic acid;
Danshensu;
Rosmarinic acid;
Salvia Miltiorrhiza Radix et Rhizoma;
Salvianolic acid B;
Salvianolic acids;
Stems and leaves of Salvia miltiorrhiza
- From:
Chinese Traditional and Herbal Drugs
2017;48(22):4688-4694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the anti-oxidative effect and chemical constituents of stems and leaves of Salvia miltiorrhiza (SMSL) collected in July and December, water extracts and alcohol extracts of SMSL collected in July and December were taken as the subject, therefore provide scientific basis for the comprehensive development and utilization of SMSL. Methods The chemical constituents in the extracts were identified and determined by Ultra-high performance liquid chromatography combined with triple quadrupole electrospray tandem mass spectrometry (UPLC-TQ/MS), then confirmed the main salvianolic acids (danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B) as the material basis of anti-oxidant activity of SMSL. Moreover, based on the anti-oxidant activity evaluation index: 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline- 6-sulphonate) (ABTS) free radical scavenging and iron reduction/anti-oxidant capacity (FRAP), anti-oxidant activity of SMSL was evaluated. Meanwhile Salvia Miltiorrhiza Radix et Rhizoma (SM) from market was used to be control. Results It showed that the water extracts of SMSL in July possessed strong anti-oxidant activities, and the total salvianolic acids with the content of 75.663 mg/g was the highest; Followed by the alcohol extracts of SMSL in July, anti-oxidant activity and total phenolic acid contents of SM extracts were all lower than that of SMSL in July. Danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B showed obvious anti-oxidative activities and significant dose-dependent effect in scavenging free radicals. Conclusion It revealed that SMSL possessed strong in vitro anti-oxidant activity. Additonally, it is shown that SMSL was rich in salvianolic acids, in which danshensu, caffeic acid, rosmarinic acid, and salvianolic acid B also had obvious anti-oxidant activity.
