Comparative analysis on contents of 10-deacetyl-bacratin III, cephalomannine, taxol of Taxus madia from different habitats and growth years
10.7501/j.issn.0253-2670.2017.23.025
- Author:
Sheng-Jun MA
1
Author Information
1. College of Food and Pharmaceutical Science, Xinjiang Agricultural University
- Publication Type:Journal Article
- Keywords:
10-deacetyl-bacratin III;
Cephalomannine;
Different habitats;
Growth years;
Taxanes;
Taxol;
Taxus madia Rehd.
- From:
Chinese Traditional and Herbal Drugs
2017;48(23):4979-4985
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analysis and compare the content differences of three effective components of taxanes of Taxus madia Rehd. from five different habitats and growth years by High Performance Liquid Chromatography (HPLC). Methods The mixed liquor of CH3OH and CHCl3 was used for the ultrasonic extraction of 10-deacetyl-bacratin III (10-DAB III), cephalomannine and taxol before being dissolved by CH3OH to obtain sample solution. By HPLC, the contents of three effective components were performed and compared. Results There were extremely significantly differences (P < 0.01) among the content of three effective components of taxanes of Taxus madia Rehd. from different habitats and growth years. Three different habitats and growth years of 10-DAB III, cephalomannine, taxol, and taxanes that have relative higher total content were HK4 > SM3 > SM2, ZL3 > HK4 > ZL4, ZL3 > SM2 > HK4, HK4 > ZL3 > ZL6, respectively. Except for the contents of 10-DAB III and cephalomannine, there were significant positive correlations among the contents of other components (P < 0.01). The contents of taxol would rise or decline when the total contents of three kinds of taxanes rised or declined among different habitats and growth years. The clustering analysis results showed that the contents of taxol have certain peculiarity. In Fuzhou of Fujian, while the total contents of three kinds of taxanes were different from others in Kaifen of Henan. Conclusion Habitants and growth years have a significant impact on the contents change of three effective components of taxanes from Taxus madia Rehd.. Therefore, regulation and control of biosynthetic pathway by choosing habitat conditions and harvest years should be considered, in order to obtain more crude drug of taxol in T. madia.
