Study on mechanism of Qinbai Qingfei Concentrated Pellets in treatment of mycoplasma pneumonia mice model based on metabolomics
10.7501/j.issn.0253-2670.2017.24.024
- VernacularTitle: 基于代谢组学的芩百清肺浓缩丸治疗支原体肺炎小鼠的作用机制研究
- Author:
Wen-Feng WEI
1
Author Information
1. Heilongjiang Academy of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Metabolomics;
Mycoplasma pneumonia;
PCA;
PLS-DA;
Qinbai Qingfei Concentrated Pellets
- From:
Chinese Traditional and Herbal Drugs
2017;48(24):5211-5216
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the mechanism of Qinbai Qingfei Concentrated Pellets (QQCP) in the treatment of mycoplasma pneumonia pneumonia. Methods: UPLC-Q-TOF-MS was applied to detect the change of endogenous substances in the lung tissue of mycoplasma pneumonia mice model after taking decoction of QQCP orally. Progenisis QI was adopted to identify and match the peak, and principal compoment analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to reduce data dimension. Through the analysis of tandem mass spectrum data of compound that had larger contribution to the separation among different groups (VIP > 1, P < 0.05) and the HMDB database retrieval to identify potential biomarkers. Results: Twenty potential biomarkers were identified from lung tissue as retinal, all-trans-retinoic acid, pyroglutamic acid, leukotriene C4, vitamin A, arachidonic acid, and prostaglandin I2. Compared with model group, QQCP group had callback function of 20 potential biomarkers. Conclusion: QQCP play a role of treatment for mycoplasma pneumonia by affecting retinol metabolism, linoleic acid, and arachidonic acid metabolism. These results indicated that QQCP had the characteristics of multi-pathway, multi-target and overall regulation in the treatment of mycoplasmal pneumoniae pneumonia.
