Genetic diversity research on Anisodus tanguticus based on ISSR molecular markers
10.7501/j.issn.0253-2670.2018.01.030
- VernacularTitle: 基于ISSR分子标记的野生山莨菪遗传多样性研究
- Author:
Dun ZHANG
1
Author Information
1. Chinese Research Academy of Environmental Science
- Publication Type:Journal Article
- Keywords:
Anisodus tanguticus (Maxim.) Pascher;
Cluster analysis;
Genetic diversity;
Geographic distance;
ISSR molecular markers
- From:
Chinese Traditional and Herbal Drugs
2018;49(1):219-226
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to provide some information for the domestication and protection of wild Anisodus tanguticus, therefore to analyze the genetic polymorphism and the relationship of A. tanguticus from Gansu and Qinghai province in China. Methods The amplifying products from 127 DNA based on ISSR makers technique were used to analyze the genetic diversity of 11 populations of wild A. tanguticus. The clustering analysis of the unweighted pair-group method with arithmetic average (UPGMA) and making Mantel test on geographic distance were carried out on the basis of genetic distance abtained, then a dendrogram was constructed. Results Total 131 alleles were amplified by using 10 ISSR primers. The number of alleles in per primer range was 11-15 with an average of 13.1, and 858 polymorphic loci were got finally. The average percentage of polymorphic loci (PPB) was 59.54%. The Nei’s gene diversity (H) and Shannon’s information index (I) were 0.220 4 and 0.325 4 respectively. The genetic distances range was 0.073 3-0.306 2, and Mantel test P = 0.002. Conclusion The genetic polymorphism among 11 populations of A. tanguticus is relatively abundant and there is a directly relationship between genetic distance and geographical distribution among them. There is a high polymorphism among A. tanguticus populations between Gansu and Qinghai and the plentiful genetic diversity of species can provide a reference for the protection and sustainable utilizing of wild A. tanguticus resources.
