AFLP analysis of genetic diversity of Aconitum brachypodum
10.7501/j.issn.0253-2670.2018.02.026
- VernacularTitle: 短柄乌头遗传多样性的AFLP分析
- Author:
Chun-Hua DU
1
Author Information
1. Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation, Yunnan Bai Yao Group Innovation and R&D Center, Yunnan Institute of Materia Medica
- Publication Type:Journal Article
- Keywords:
Aconitum brachypodum Diels;
AFLP;
Cluster analysis;
Genetic diversity;
Germplasm resources
- From:
Chinese Traditional and Herbal Drugs
2018;49(2):439-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genetic diversity of Aconitum brachypodum, an endangered medicinal plants. Methods Three pair primers were used to evaluate the relevant genetic parameters and clustering pattern for four natural populations containing 105 individuals by using POPGENE32, MEGA4, NTSYS and other biometric softwares. Results For A. brachypodum, the Nei’s genetic diversity index (He), Shannon’s information index (I), percentage of polymorphic loci (PPB) were 0.322 9 ± 0.179 8, 0.472 0 ± 0.251 7, and 80.57%, respectively; and the population level were 0.115 4 ± 0.044, 0.168 0 ± 0.065 3, and 12%, respectively. The genetic differentiation index (Gst) was 0.864 2. The clustering results showed that, Luquan and Dongchuan populations are grouped together, Lijiang and Huize populations are clustered together. Conclusion A.brachypodum possesses the relatively high level of genetic diversity at the species level, however, low within populations. This study should provide an initial insights for A.brachypodum germplasm conservation
