Origin of cultivated Rehmannia glutinosa based on chloroplast gene psbA-trnH, trnS-trnG, and nuclear ITS sequences
10.7501/j.issn.0253-2670.2018.02.024
- Author:
Zhi XIA
1
Author Information
1. College of Agronomy, Henan Agricultural University
- Publication Type:Journal Article
- Keywords:
Internal transcibed spacer (ITS);
Origin;
PsbA-trnH;
Rehmannia glutinosa (Gaert.) Libosch. ex Fisch;
TrnS-trnG
- From:
Chinese Traditional and Herbal Drugs
2018;49(2):423-430
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to understand the genetic diversity of cultivated and wild Rehmannia glutinosa, and to unravel the origin of cultivated R. glutinosa. Methods The sequences of nuclear gene ITS and chloroplast gene psbA-trnH, trnS-trnG in cultivar and wild population of R. glutinosa were amplified and sequenced. Haplotype (gene) diversity and nucleotide polymorphism of three genes from wild and cultivars of R. glutinosa were analyzed and compared in this study. Phylogenetic tree was constructed based on combined three genes using Bayesian inference (BI) methods. Results Analysis of sequences indicated that the number of haplotype (ITS, 6; psbA-trnH, 8; trnS-trnG, 9) in wild population of R. glutinosa was obviously higher than the number of Haplotype (ITS, 3; psbA-trnH, 3; trnS-trnG, 3) in cultivars of R. glutinosa. Haplotype diversity and nucleotide polymorphism of wild population of R. glutinosa were far higher than that of cultivars of R. glutinosa. The NJ tree (combined three genes data) indicated that all cultivated and wild population of R. glutinosa, and R. solanifolia form a monophyletic clade [Posterior probability (PP) = 90%]. Twenty-three cultivars of R.glutinosa (including 29 samples) were clustered with Wenxian wild populations (PP = 78%). Conclusion The results implied very low genetic diversity existed in cultivars of R. glutinosa induced by the severe genetic bottleneck during the process of domestication of wild R. glutinosa, which resulted in the narrow genetic basis of the existing cultivars and decreased genetic diversity. Furthermore, it appeared that wild populations in Wenxian-Henan area were involved in the origin of cultivars of R. glutinosa.
