Cloning of BpTRX gene and promoter and expression pattern analysis under H2S treatment in Betula platyphylla
10.7501/j.issn.0253-2670.2018.02.023
- Author:
Zi-Qing WANG
1
Author Information
1. College of Life Sciences, Northeast Forestry University
- Publication Type:Journal Article
- Keywords:
Betula platyphylla Suk.;
Bioinformatic analysis;
Hydrogen sulfide;
Quantitative real-time PCR;
Thioredoxin
- From:
Chinese Traditional and Herbal Drugs
2018;49(2):414-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the full-length, promoter sequences and its coding structure and properties of thioredoxin (Trx) gene of Betula platyphylla (BpTRX), and reveal the expression pattern of BpTRX under H2S treatment. Methods The BpTRX gene was cloned by PCR, and the promoter region sequences of BpTRX was obtained by using chromosome walking technique. The BpTRX gene, the promoter and its encoded protein were analyzed by bioinformatics software. The phylogenetic tree of BpTRX was constructed. The expression patterns of BpTRX gene under H2S exogenous stress were analyzed by quantitative real-time PCR. Results The full-length of BpTRX gene is 351 bp, encoding 117 amino acids. The BpTRX gene was closely related to the TRX-H protein of castor bean, soybean, alfalfa and grape. The obtained BpTRX promoter region sequences are 873 bp, which contains the essential elements of transcription and a large number of stress response and hormone response elements. Conclusion The full length and partial promoter sequences of the BpTRX gene were obtained. The response trend of BpTRX gene to H2S treatment was in the form of bimodal, which was first rises, then decreases, then rises and then declines.
