Study on preparation and storage stability of ginsenoside Re liposomes
10.7501/j.issn.0253-2670.2018.02.011
- Author:
Jin-Zhi GAO
1
Author Information
1. College of TCM Materials, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Entrapment efficiency;
Freezing-drying technology;
Ginsenoside Re;
Liposomes;
Orthogonal test;
Storage stability
- From:
Chinese Traditional and Herbal Drugs
2018;49(2):330-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and optimize preparation technology of ginsenoside Re liposomes, therefore to improve storage stability. Methods Ginsenoside Re liposomes were prepared by the method of film dispersion-mechanical vibration, which were collected by separating liposome from disclosed free drug by dialysis method. Measure entrapment efficiency by HPLC. Prepare freeze-dried liposome preparations by freezing-drying technology. Taking entrapment efficiency as the main screening index, optimize liposome formulation and freezing-drying technology by orthogonal test design. Results The entrapment efficiency of ginsenoside Re lipidosomes prepared by the method of film dispersion-mechanical vibration is the highest. The best formulation technology is: Mass ratio of drug and phospholipid is 1∶30, mass ratio of phospholipid and cholesterol is 16∶1, ice-water bath ultrasound is 30 min, and double distilled water is hydration solution; The best freezing-drying technology is: Taking sucrose as the freeze-drying protective agent, mass ratio of disaccharide-water is 1∶10, pre-freezing temperature is −20 ℃, and normal saline of 0.9% is reconstitution solution. Conclusion The preparation technology of liposome is stable and practicable. The ginsenoside Re liposome prepared by taking the sucrose as the freeze-drying protective agent has good indexes, which can extend the storage period.
