Study on HPLC specific chromatograms of effective parts and determination of five components of Filifolium sibiricum
10.7501/j.issn.0253-2670.2018.05.026
- Author:
Shao-Wa LV
1
Author Information
1. Heilongjiang Provincial Key Laboratory of Traditional Chinese Medicine and Effective Substance Basis of Natural Medicine, Key Laboratory of Basis and Application Research of North Medicine, Heilongjiang University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Determination;
Effective parts of Filifolium sibiricum;
HPLC specific chromatograms;
Isoquercitrin;
Isorhamnetin-3-O-β-D-glucose;
Isorientin;
Isovitexin;
Luteoloside
- From:
Chinese Traditional and Herbal Drugs
2018;49(5):1160-1164
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the HPLC specific chromatograms of the ethyl acetate layer in ten batches of effective parts of Filifolium sibiricum and to determine the contents of five components. Methods: The analysis of effective parts of F. sibiricum was performed on a Thermo AcclaimTM120 C18 column (250 mm × 4.6 mm, 5 μm) with acetonitrile (B)-PBS (A) (0.1 mol/L sodium dihydrogen phosphate-2% glacial acetic acid, 1∶1) as the mobile phase in a gradient elution mode, the detection wavelength was set at 360 nm, the flow rate was 0.8 mL/min, and the column temperature was 35 ℃. Results: The specific chromatograms of F. sibiricum effective parts were established and ten common peaks were designated. Among them, five components including isorientin, isovitexin, isoquercitrin, luteoloside and isorhamnetin-3-O-β-D-glucose all showed good linear relationship within the ranges of 0.018—0.108, 0.066 8—0.400 8, 0.088—0.528, 0.118 4—0.710 4 and 0.017 6—0.105 6 μg, respectively. The average recovery was 98.67%, 97.93%, 98.95%, 99.81%, and 97.33% with the RSD value at 1.10%, 0.93%, 1.10%, 0.62%, and 1.48%, respectively. Moreover, the similarity of the eight batches of samples was above 0.9 in the ten batches of medicinal herbs, the similarity of the two batches of which was 0.688 and 0.695, indicating that its content was lower and the difference was greater. In addition, there were significant differences in the content of five components in each harvest time. The content of flavonoids in medicinal herbs was higher with high flower percentage. It was suggested that the content of flavonoids in F. sibiricum was related to the flower percentage of harvest period. Conclusion: The HPLC specific chromatograms of the F. sibiricum effective parts were established and the common characteristic peaks were determined, which could be used for quality control of the F. sibiricum.
