Study on transformation of ginsenoside Rg3 fermented by Monascus purpureus
10.7501/j.issn.0253-2670.2018.06.010
- Author:
Yue-Yi CONG
1
Author Information
1. College of Life Science, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Biotransformation;
Ginsenoside Rg3;
HPLC;
Monascus purpureus Went;
Panax ginseng C. A. Mey.;
Solid fermentation;
Static dark culture method;
Total saponins
- From:
Chinese Traditional and Herbal Drugs
2018;49(6):1298-1303
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the solid fermentation process of Panax ginseng by Monascus purpureus, which can transfer some major ginsenoside into rare ginsenoside Rg3 with stronger biological activity. Methods: The static dark culture method was used to perform microbial fermentation; Vanilline-glacial acetic acid method was used to detect the total saponins before and after fermentation, and the ginsenoside Rg3 was detected by HPLC. Results: The optimum process parameters of Monascus purpureus fermentation was fermentation 6 d, fermentation temperature 32 ℃, pH 7.0, and water content of substrate 50%. After 6 d of fermentation, the content of total saponins in fermentation products increased by 40%, and the content of ginsenoside Rg3 was 6.047 mg/g, which was 2.3 times as much as that of non-fermented P. ginseng. According to the change of monomer saponin content along with the fermentation time, it was deduced that the transformation path was Rb1 (Rb2)→Rd→Rh2→Rg3. Conclusion: The solid fermentation process of Monascus purpureus established in this study is reasonable, which not only lays a foundation for the directional production of rare saponins Rg3 but provides a theoretical support for preparing rare ginsenoside in vitro.
