Biotransformation and antitumor activity of triterpenoid derivatives from Ardisia gigantifolia
10.7501/j.issn.0253-2670.2018.06.006
- Author:
Li-Hua MU
1
Author Information
1. Department of Clinical Pharmacology, General Hospital of PLA
- Publication Type:Journal Article
- Keywords:
3β-O-{β-D-gluco-pyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranoside}-cyclamiretin A;
Antitumor;
Ardisia gigantifolia Stapf.;
Biotransformation;
Cyclamiretin A;
Endophyte;
Enzyme;
Triterpenoid saponins
- From:
Chinese Traditional and Herbal Drugs
2018;49(6):1266-1271
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents of transformed products by Sphingomonas yabuuchiae GTC 868T (AB071955) and Pectinex Ultra AFP from the saponin of Ardisia gigantifolia. Methods: Transformation products separated by the process of silica gel column, compounds were identified and elucidated by spectral and chemical methods. Their cytotoxicity activities were tested by Cell Counting Kit 8 colorimetric assay. Results: Five triterpenoid saponins were obtained, including 3β-O-{α-L-rhamnopyranosyl-(1→3)-[β-D-xylopyranosyl-(1→2)]-β-D-glucopyranosyl-(1→4)-α-L-arabinopyranosyl}-cyclamiretin A (1), 3β-O-{β-D-glucopyranosyl-(1→4)-[β-D-glucopyranosyl-(1→2)]-α-L-arabinopyranoside}-cyclamiretin A (2), 3β-O-{β-D-glucopyranosyl-(1→2)-α-L-arabinopyranoside}-cyclamiretin A (3), 3-O-α-L-arabinopyranosyl cyclamiretin A (4), and cyclamiretin A (5). Conclusion: Compounds 2-5 are obtained by biotransformation for the first time. Some of the compounds showed certain antitumor activity, among them, compound 2 shows more cytotoxicity activity than Ag3 and positive control.
